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Vibeke Jensen

Hormone receptor analysis: NordiQC results.

Handout

Immunohistochemical quality control have included ER and PgR since 2003/2005. Until 2013 there have been 12 assessments of ER and 7 assessments of PgR. The number of participants has increased from 71 in 2003 to 262 in 2013. The presentation concerns criteria for classification staining results as sufficient (optimal/good) and insufficient (borderline/poor). During the 10 years, the pass rate for ER has increased from 45% to 82% and the pass rate for PgR has increased from 69% to 87%. This increase has been relatively constant through the 10 year period. A small difference in pass rates is observed for laboratories participating the assessment for the first time compared to laboratories participating in two or more assessments. From data in the NordiQC database it is evident that changing the protocols according to given recommendations most often improves staining results from insufficient to sufficient.

Examples of optimal and insufficient staining results will be given and some of the most common reasons for insufficient staining will be highlighted. Insufficient HIER (too short efficient HIER time) and too low concentration of the primary antibody are the most common causes of borderline/poor staining results. The most robust antibodies in the last assessment for ER were clones 1D5, 6F11, EP1 and SP1, and for PgR the clones16 and Pgr636 showed the highest pass rate.

Finally - recommendations for tissues to be used as positive and negative controls in daily laboratory practice will be suggested.

CV

Vibeke Jensen, MD, Ph.d., senior consultant at Department of Pathology, Aarhus Universisity Hospital, Denmark.
Dr. Jensen is breast pathologist and member of the NordiQC Breast Cancer IHC Assessor Group since 2006.

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